Phosphorylation mechanisms in dopamine transporter regulation
نویسندگان
چکیده
منابع مشابه
Different Binding Mechanisms of Dopamine Transporter Inhibitors
DAT inhibitors have at least two different binding mechanisms (Scheme 1): fast ligand binding following the conventional equilibrium scheme (i) and their binding effectiveness is described by the KA values; two-step binding, after initial fast binding step the ligand induces protein conformation change (ii) [2]. This extra step consists of a slow protein conformational change, enhancing the app...
متن کاملPhosphorylation of dopamine transporter serine 7 modulates cocaine analog binding.
As an approach to elucidating dopamine transporter (DAT) phosphorylation characteristics, we examined in vitro phosphorylation of a recombinant rat DAT N-terminal peptide (NDAT) using purified protein kinases. We found that NDAT becomes phosphorylated at single distinct sites by protein kinase A (Ser-7) and calcium-calmodulin-dependent protein kinase II (Ser-13) and at multiple sites (Ser-4, Se...
متن کاملMechanisms underlying methamphetamine-induced dopamine transporter complex formation.
Repeated, high-dose methamphetamine (METH) administrations cause persistent dopaminergic deficits in rodents, nonhuman primates, and humans. In rats, this treatment also causes the formation of high-molecular mass (greater than approximately 120 kDa) dopamine transporter (DAT)-associated complexes, the loss of DAT monomer immunoreactivity, and a decrease in DAT function, as assessed in striatal...
متن کاملPhysicochemical mechanisms of protein regulation by phosphorylation
Phosphorylation offers a dynamic way to regulate protein activity and subcellular localization, which is achieved through its reversibility and fast kinetics. Adding or removing a dianionic phosphate group somewhere on a protein often changes the protein's structural properties, its stability and dynamics. Moreover, the majority of signaling pathways involve an extensive set of protein-protein ...
متن کاملDopamine D2 receptor regulation of the dopamine transporter expressed in Xenopus laevis oocytes is voltage-independent.
Presynaptic dopamine D2 receptors (D2Rs) regulate dopamine transporter (DAT) activity in the brain. A potential mechanism was suggested by the observations that somatodendritic D2R activation produces hyperpolarization and the velocity of DAT expressed in Xenopus laevis oocytes varies with changes in membrane potential. To investigate whether D2R regulation of DAT function is voltage-dependent,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chemical Neuroanatomy
سال: 2017
ISSN: 0891-0618
DOI: 10.1016/j.jchemneu.2016.10.004